Modernization of analytical methods to characterize different forms and metabolites of B-vitamins
Start date: 1. January 2017
End date: 31. December 2017
Financed by: NIFES
The project is lead by: NIFES

Background

The eight B-vitamins (B1, B2, B3, B5, B6, B7, B9, B12) are essential nutrients for fish and humans and function e.g. as co-enzymes in cellular metabolism and energy production. They are contributing to the maintenance of building blocks such as DNA, proteins, carbohydrates and lipids, and are necessary for the production of red blood cells and normal function of the nervous system. Adequate availability of the B-vitamins is thus necessary for good health.
In current fish farming the feed often originates from plants which might result in too low accessibility of B-vitamins, and supplementation might be necessary for good apatite and growth. Sufficient amount of B-vitamins in fish is also important since fish as food is a natural B-vitamin source. In a healthy diet the B-vitamins are normally physiologically balanced, but this should be better characterized in different life situations.
Current microbiology and fluorescence methods are inadequate to analyze all the various forms and metabolites of the B-vitamins. A priority area of research at NIFES is therefore modernization of the methods by developing a vitaminomics strategy to characterize many forms and metabolites of the B-vitamins in one analysis. For this purpose we apply ultra-high performance liquid chromatography coupled to high resolution accurate mass and triple quadrupole mass spectrometers.

Objective

Modernization of analytical methods to characterize the different forms and metabolites of B-vitamins in feed, nutrients, tissue and tissue fluids related to research projects in fish- and human nutrition.

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